Cytometric study of the female Syrian hamster gallbladder epithelium following sex steroid administration.

Title

Cytometric study of the female Syrian hamster gallbladder epithelium following sex steroid administration.

Creator

Adamiec-Beyga E; Karkare S; Kelly T R; Gilloteaux J

Publisher

Tissue & cell

Date

1993
1993-08

Description

This report is a cytometric study of the female Syrian hamster gallbladder epithelium following 1-, 2-, and 3-month administration of female sex steroids. Nulliparous, multiparous, young, old and pregnant hamsters were used in this study. A 1 month treatment with estrogen alone significantly increases the nuclear volume of the gallbladder epithelial cells, while E + P treatment significantly affects the nuclear volume only after a 2 month treatment. On the other hand, E + P and P treatments significantly increase the cell volumes as compared to the E-treated groups, this effect is most striking following the 1 month period. Prolonged sex steroid treatment (2 and 3 month) does not appear to influence the gallbladder epithelial cell and nuclear volumes as dramatically as that observed following the 1 month treatment. The nulliparous, progesterone-treated hamsters appear to have a greater cytoplasmic volume than the multiparous group and this is substantiated by the bulging apices and the luminal cellular excrescences observed with scanning and transmission electron microscopy. These observations are similar to those reported in ovariectomized hamsters (Gilloteaux et al., 1992). Further, the gallbladder epithelial cells and nuclei of the older female hamsters demonstrate an accentuated response to a 1 month sex steroid treatment as compared to the younger hamsters for the same treatment duration. These results enable us to hypothesize that changes induced by a short term sex steroid treatment participate in the gallstone nucleation process, while longer duration of the treatments contribute to progressive enlargement and accumulation of gallbladder calculi.

Subject

Animals; Cell Count; Cholelithiasis/*chemically induced/pathology; Cricetinae; Cytoplasm/drug effects; Drug Administration Schedule; Epithelial Cells; Epithelium/drug effects; Estrogens/*pharmacology; Female; Gallbladder/cytology/*drug effects; Mesocricetus; Progesterone/*pharmacology

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

527–536

Issue

4

Volume

25

Citation

Adamiec-Beyga E; Karkare S; Kelly T R; Gilloteaux J, “Cytometric study of the female Syrian hamster gallbladder epithelium following sex steroid administration.,” NEOMED Bibliography Database, accessed May 12, 2021, https://neomed.omeka.net/items/show/3314.

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