Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1beta-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte.

Title

Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1beta-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte.

Creator

Makki Mohammad Shahidul; Haqqi Tariq M

Publisher

The American journal of pathology

Date

2016
2016-10

Description

Osteoarthritis (OA) is the most common whole-joint disease and is characterized by progressive loss of the cartilage matrix. Matrix metallopeptidase-13 (MMP-13) is a highly active and an abundantly expressed protease in OA cartilage and chondrocytes and degrades type II collagen and proteoglycans. We investigated the mechanism of MMP-13 suppression by histone deacetylase inhibitor vorinostat (SAHA). OA chondrocytes were obtained from knee cartilage after enzymatic digestion and treated with IL-1beta in the absence or presence of various histone deacetylase inhibitors. Gene expression was quantified using quantitative RT-PCR. Protein expression and chromatin modifications were determined by Western immunoblotting using specific antibodies. The effect of IL-6 on the expression of

Subject

Aged; Articular/drug effects/metabolism; Blotting; Cartilage; Chondrocytes/drug effects; Collagen Type II/drug effects/metabolism; Down-Regulation/drug effects; Female; Gene Expression Regulation/*drug effects; Histone Deacetylase Inhibitors/*pharmacology; Humans; Hydroxamic Acids/*pharmacology; Interleukin-1beta/*antagonists & inhibitors/genetics/metabolism; Knee Joint/metabolism; Male; Matrix Metalloproteinase 13/*drug effects/metabolism; Middle Aged; Osteoarthritis/*drug therapy; Proteoglycans/metabolism; Tumor Necrosis Factor-alpha/drug effects/metabolism; Vorinostat; Western

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

2701–2708

Issue

10

Volume

186

Citation

Makki Mohammad Shahidul; Haqqi Tariq M, “Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1beta-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte.,” NEOMED Bibliography Database, accessed September 28, 2021, https://neomed.omeka.net/items/show/3443.

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