Pregnancy zone protein is a carrier and modulator of placental protein-14 in

Title

Pregnancy zone protein is a carrier and modulator of placental protein-14 in

Creator

Skornicka Erin L; Kiyatkina Nadya; Weber Matthew C; Tykocinski Mark L; Koo Peter H

Publisher

Cellular immunology

Date

2004
2004-12

Description

A successful pregnancy can only occur when the maternal immune system fails to attack the allogeneic fetus. Two plasma proteins with described immunoregulatory activities, pregnancy zone protein (PZP) and placental protein-14 (PP14; also known as glycodelin-A), increase dramatically during pregnancy, prompting us to examine their potential role in mediating fetal protection. First, we demonstrated that both native PZP and its receptor-recognized monoamine-activated form (MA-PZP) bound non-covalently and specifically to PP14, exhibiting K(d) values greater than 3 microM, as determined by surface plasmon resonance. Our evidence further suggests that PZP is potentially a more effective carrier of PP14 than its relative alpha2-macroglobulin. Second, we found that T-cell activation, as measured by increased proliferation and IL-2 production, was inhibited by either PZP or PP14 in a dose-dependent manner. However, when PZP and PP14 were combined, they acted synergistically to inhibit T cell proliferation and IL-2 production. Interestingly, the combination of PZP and PP14 had little effect on the production of T(H)2 cytokine, IL-4. Based upon these findings, we hypothesize that PZP and PP14 form a stable complex in the plasma of pregnant women and together act synergistically to selectively modulate T-cell activation. Mechanistically, this activity appears to be independent of the PZP receptor (CD91) or PZP's anti-proteinase activity.

Subject

alpha-Macroglobulins/immunology/metabolism; Antigens; Carrier Proteins/immunology/metabolism/*physiology; CD/drug effects/immunology; Cell Proliferation/drug effects; Cytokines/*biosynthesis/drug effects; Female; Glycodelin; Glycoproteins/immunology/metabolism/*physiology; Humans; Interleukin-2/biosynthesis; Interleukin-4/biosynthesis; Kinetics; Leukocytes; Low Density Lipoprotein Receptor-Related Protein-1; Lymphocyte Activation/immunology; Mononuclear/drug effects/immunology; Pregnancy; Pregnancy Proteins/immunology/metabolism/*physiology; Protein Binding; T-Lymphocytes/cytology/drug effects/*immunology; Time Factors

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

144–156

Issue

1

Volume

232

Citation

Skornicka Erin L; Kiyatkina Nadya; Weber Matthew C; Tykocinski Mark L; Koo Peter H, “Pregnancy zone protein is a carrier and modulator of placental protein-14 in,” NEOMED Bibliography Database, accessed September 24, 2022, https://neomed.omeka.net/items/show/3520.

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