Maturation of dendritic cell precursors into IL12-producing DCs by J-LEAPS immunogens.

Maturation of dendritic cell precursors into IL12-producing DCs by J-LEAPS immunogens.

Taylor Patricia R; Paustian Christopher C; Koski Gary K; Zimmerman Daniel H; Rosenthal Ken S

Cellular immunology

2010

1905-07

LEAPS (ligand epitope antigen presentation system) vaccines consist of a peptide containing a major histocompatibility antigen binding peptide conjugated to an immune cell binding ligand (ICBL) such as the 'J' peptide from beta-2-microglobulin. Treatment of monocytes, monocytes plus GMCSF, or monocytes plus GMCSF and IL4 with JgD (containing a peptide from gD of herpes simplex virus type 1) or JH (with a peptide from HIV p17 gag protein) was sufficient to promote their maturation into Interleukin 12 producing dendritic cells. JgD-dendritic cells supported allotypic activation of T cells to produce Th1-related cytokines.

*Antigen Presentation; *Cell Differentiation; Coculture Techniques; Dendritic Cells/*cytology/*immunology; Epitopes/*immunology; Humans; Immunoglobulin J-Chains/*immunology; Interleukin-12/*biosynthesis/immunology

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

1–5

1

262