Effects of FGF-2 and OP-1 in vitro on donor source cartilage for auricular reconstruction tissue engineering.

Effects of FGF-2 and OP-1 in vitro on donor source cartilage for auricular reconstruction tissue engineering.

Shasti Mark; Jacquet Robin; McClellan Phillip; Yang Julianne; Matsushima Seika; Isogai Noritaka; Murthy Ananth; Landis William J

International journal of pediatric otorhinolaryngology

2014

2014-03

OBJECTIVE: Microtia is a congenital partial or total loss of the external ear with current treatment approaches involving autologous construction from costal cartilage. Alternatively, tissue engineering provides possible use of normal or microtia auricular chondrocytes harvested from patients. This study investigated effects in vitro of basic fibroblast growth factor (FGF-2) and osteogenic protein 1 (OP-1) on human pediatric normal and microtia auricular chondrocytes and their potential proliferation and differentiation for cellular expansion. A working hypothesis was that FGF-2 promotes proliferation and OP-1 maintains an auricular phenotype of these cells. METHODS: Two patients, one undergoing otoplasty and one an ear construction, yielded normal and microtia auricular chondrocytes, respectively. The two donor sets of isolated chondrocytes were equally divided into four experimental cell groups. These were controls without added growth factors and cells supplemented with FGF-2, OP-1 or FGF-2/OP-1 combined. Cells were cultured 3, 5, 7, and 10 days (3 replicates/time point), counted and assayed by RT-qPCR to determine elastin and types II and III collagen gene expression. RESULTS: Compared to control counterparts, normal and microtia chondrocytes with

Bone Morphogenetic Protein 7/*pharmacology; Cell Differentiation/drug effects; Cells; Child; Chondrocytes; Chondrocytes/*drug effects/physiology; Congenital Abnormalities/diagnosis/*surgery; Congenital Microtia; Cultured; Ear Cartilage/surgery; Ear/*abnormalities/surgery; FGF-2; Fibroblast Growth Factor 2/*pharmacology; Humans; Male; Microtia; OP-1; Reconstructive Surgical Procedures/*methods; Reference Values; Tissue Donors; Tissue engineering; Tissue Engineering/*methods

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