A rapid UPLC-MS/MS assay for eicosanoids in human plasma: Application to evaluate niacin responsivity.

Title

A rapid UPLC-MS/MS assay for eicosanoids in human plasma: Application to evaluate niacin responsivity.

Creator

Miller Tricia M; Poloyac Samuel M; Anderson Kacey B; Waddell Brooke L; Messamore Erik; Yao Jeffrey K

Publisher

Prostaglandins, leukotrienes, and essential fatty acids

Date

2018
2018-09

Description

A rapid and sensitive method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to simultaneously quantify hydroxyeicosatetraenoic (HETE), dihydroxyeicosatrienoic (DiHETrE), epoxyeicosatrienoic acid (EET), and prostaglandin metabolites of arachidonic acid in human plasma. Sample preparation consisted of solid phase extraction with Oasis HLB (30mg) cartridges for all metabolites. Separation of HETEs, EETs, and DiHETrEs was achieved on an Acquity UPLC BEH C18, 1.7microm (100x2.1mm) reversed-phase column (Waters Corp, Millford, MA) with negative electrospray ionization mass spectrometric detection. A second injection of the same extracted sample allowed for separation and assessment of prostaglandin metabolites under optimized UPLC-MS/MS conditions. Additionally, the endogenous levels of these metabolites in five different matrices were determined in order to select the optimal matrix for assay development. Human serum albumin was shown to have the least amount of endogenous metabolites, a recovery efficiency of 79-100% and a matrix effect of 71 - 100%. Linear calibration curves ranging from 0.416 to 66.67ng/ml were validated. Inter-assay and intra-assay variance was less than 15% at most concentrations. This method was successfully applied to quantify metabolite levels in plasma samples of healthy control subjects receiving niacin administration to evaluate the association between niacin administration and eicosanoid plasma level response.

Subject

Arachidonic acid; Eicosanoids; Mass spectrometry; Niacin; Plasma; Prostanoids; Schizophrenia; Ultra performance liquid chromatography; UPLC-MS/MS

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

153–159

Volume

136

Citation

Miller Tricia M; Poloyac Samuel M; Anderson Kacey B; Waddell Brooke L; Messamore Erik; Yao Jeffrey K, “A rapid UPLC-MS/MS assay for eicosanoids in human plasma: Application to evaluate niacin responsivity.,” NEOMED Bibliography Database, accessed June 13, 2021, https://neomed.omeka.net/items/show/3774.

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