J-LEAPS vaccines initiate murine Th1 responses by activating dendritic cells.

Title

J-LEAPS vaccines initiate murine Th1 responses by activating dendritic cells.

Creator

Taylor P R; Koski G K; Paustian C C; Bailey E; Cohen P A; Moore F B-G; Zimmerman D H; Rosenthal K S

Publisher

Vaccine

Date

2010
2010-08

Description

The Ligand Epitope Antigen Presentation System (LEAPS) converts a peptide containing a T cell epitope as small as 8 amino acids into an immunogen and directs the nature of the subsequent response. Tandem synthesis of the J peptide (a peptide from the beta-2-microglobulin) with peptides of 15 or 30 amino acids from HSV-1 or HIV made them immunogenic and promoted Th1 immune responses. Immunization of A/J or C57BL/6 mice with J-LEAPS heteroconjugates containing an epitope from the HSV-1 glycoprotein D (JgD) or an epitope from the HIV gag protein (JH) emulsified with Seppic ISA51 induced increased levels of IL-12p70 by day 3 and increased levels of interferon gamma (IFN-gamma) on days 10 and 24. Interestingly, levels of IL-10, TNF-alpha, and IL-6 did not change. Neither the H nor the gD peptides alone elicited responses and only weak responses followed immunization with the J peptide. Bone marrow (BM) cells became CD86 and CD11c positive within 48 h of treatment with JgD or JH. JH or JgD treatment promoted

Subject

*Antigen Presentation; Adoptive Transfer; Animals; Cells; Cultured; Dendritic Cells/*immunology; Epitopes; Female; gag Gene Products; Herpes Simplex/immunology/prevention & control; Herpesvirus 1; Human Immunodeficiency Virus/immunology; Human/immunology; Inbred A; Inbred C57BL; Interferon-gamma/immunology; Interleukin-12/immunology; Mice; T-Lymphocyte/immunology; Th1 Cells/*immunology; Viral Envelope Proteins/immunology; Viral Vaccines/*immunology

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

5533–5542

Issue

34

Volume

28

Citation

Taylor P R; Koski G K; Paustian C C; Bailey E; Cohen P A; Moore F B-G; Zimmerman D H; Rosenthal K S, “J-LEAPS vaccines initiate murine Th1 responses by activating dendritic cells.,” NEOMED Bibliography Database, accessed December 3, 2021, https://neomed.omeka.net/items/show/3814.

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