Flow cytometric and ultrastructural aspects of the synergistic antitumor activity of vitamin C-vitamin K3 combinations against human prostatic carcinoma cells.

Title

Flow cytometric and ultrastructural aspects of the synergistic antitumor activity of vitamin C-vitamin K3 combinations against human prostatic carcinoma cells.

Creator

Jamison J M; Gilloteaux J; Venugopal M; Koch J A; Sowick C; Shah R; Summers J L

Publisher

Tissue & cell

Date

1996
1996-12

Description

Transmission and scanning electron microscopy and flow cytometry were employed to characterize the cytotoxic effects of vitamin C (VC), vitamin K3 (VK3), or VC-VK3 combinations on a human prostate carcinoma cell line (DU145) following a 1-h vitamin treatment and a 24-h incubation in culture medium. Cells exposed to VC exhibited membranous blebs, aberrant microvillar morphology, mitochondria with swollen cristae and intramitochondrial deposits, as well as nucleoli with segregated components. VK3-treated cells displayed a damaged cytoskeleton and membranes, a cytoplasm which contained large lumen, condensed polysomes, and severely damaged mitochondria with residual bodies, and nuclei which exhibited chromatic condensation, pyknosis, and karyolysis. VC-VK3-treated cells exhibited characteristics consistent with necrosis, i.e. swollen mitochondria and swollen, achromatic nuclei with marginated chromatin and intact envelopes, while other cells displayed characteristics consistent with apoptosis, i.e. expulsion of organelle-containing blebs, margination of nuclear chromatin, and segregation of nucleolar components. Vitamin treatment also decreased DNA synthesis, induced a S/G2 block in the cell cycle, and resulted in the accumulation of fragmented DNA. These results suggested that increased oxidative stress, subsequent membrane damage, and DNA fragmentation were responsible for enhanced cytotoxicity of the vitamin combination.

Subject

*Prostatic Neoplasms; Antineoplastic Combined Chemotherapy Protocols/*pharmacology; Ascorbic Acid/administration & dosage; Cultured/drug effects/metabolism/ultrastructure; DNA/biosynthesis; Drug Synergism; Electron; Flow Cytometry; Hemostatics/administration & dosage; Humans; Male; Microscopy; Scanning; Thymidine/metabolism; Tritium; Tumor Cells; Vitamin K/administration & dosage

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

687–701

Issue

6

Volume

28

Citation

Jamison J M; Gilloteaux J; Venugopal M; Koch J A; Sowick C; Shah R; Summers J L, “Flow cytometric and ultrastructural aspects of the synergistic antitumor activity of vitamin C-vitamin K3 combinations against human prostatic carcinoma cells.,” NEOMED Bibliography Database, accessed October 20, 2021, https://neomed.omeka.net/items/show/3866.

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