Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.

Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.

Schechter J B; Crisp T

Journal of neuroscience methods

2000

2000-04

In order to identify an acute spinally mediated pharmacological effect of a bioactive substance, without incurring untoward supraspinal effects, it is necessary to administer the agent locally onto the spinal cord. The procedure delineated herein presents a modern technique to install a stable, permanent indwelling thecal cannulae with a cranially mounted aperture, and details a simple, repeatable administration system. These methods facilitate a quick, noninvasive spinal drug microadministration that is most useful for differentiation of the locus of pharmacological action without the behavioral disruption associated with other administration methodologies.

Analgesics; Anesthesia; Animals; Dose-Response Relationship; Drug; Injections; Microinjections; Morphine/administration & dosage/pharmacology; Opioid/administration & dosage/pharmacology; Rats; Spinal Cord/*physiology; Spinal/*instrumentation; Sprague-Dawley; Stereotaxic Techniques/*instrumentation

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