Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.

Title

Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.

Creator

Schechter J B; Crisp T

Publisher

Journal of neuroscience methods

Date

2000
2000-04

Description

In order to identify an acute spinally mediated pharmacological effect of a bioactive substance, without incurring untoward supraspinal effects, it is necessary to administer the agent locally onto the spinal cord. The procedure delineated herein presents a modern technique to install a stable, permanent indwelling thecal cannulae with a cranially mounted aperture, and details a simple, repeatable administration system. These methods facilitate a quick, noninvasive spinal drug microadministration that is most useful for differentiation of the locus of pharmacological action without the behavioral disruption associated with other administration methodologies.

Subject

Analgesics; Anesthesia; Animals; Dose-Response Relationship; Drug; Injections; Microinjections; Morphine/administration & dosage/pharmacology; Opioid/administration & dosage/pharmacology; Rats; Spinal Cord/*physiology; Spinal/*instrumentation; Sprague-Dawley; Stereotaxic Techniques/*instrumentation

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

31–35

Issue

1

Volume

97

Citation

Schechter J B; Crisp T, “Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.,” NEOMED Bibliography Database, accessed September 29, 2022, https://neomed.omeka.net/items/show/3882.

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