Modulation of olfactory bulb tyrosine hydroxylase and catecholamine transporter mRNA by estrogen.


Modulation of olfactory bulb tyrosine hydroxylase and catecholamine transporter mRNA by estrogen.


Dluzen Dean E; Park June-Hee; Kim Kyungjin


Brain research. Molecular brain research




Since estrogen exerts wide ranging effects within the central nervous system, it is important to investigate the sites and actions of this gonadal steroid hormone at extra-hypothalamic locations. In the present report, the effects of estrogen upon catecholaminergic function within the olfactory bulb were examined. To assess the role of estrogen at this site, ovariectomized mice received either no further hormonal treatment or were treated with estrogen, the anti-estrogen, tamoxifen, or a combination of estrogen and tamoxifen as administered in a 21-day release pellet. At 14 days post-hormonal treatment, the olfactory bulbs were assayed for mRNA levels of tyrosine hydroxylase, dopamine transporter and norepinephrine transporter using competitive-PCR. Tyrosine hydroxylase mRNA levels in either estrogen or estrogen+tamoxifen treated females were significantly decreased compared with non-hormonally treated controls. In addition, tyrosine hydroxylase mRNA levels of tamoxifen-treated mice were significantly greater than that of estrogen-treated mice. Dopamine transporter mRNA levels of tamoxifen-treated females were significantly greater than that of non-hormonally treated controls and estrogen treated mice. The combination of estrogen+tamoxifen significantly increased dopamine transporter mRNA levels compared to that of estrogen treated mice. No overall statistically significant differences in norepinephrine transporter mRNA levels were obtained among the four treatment groups. The data demonstrate that estrogen can exert significant modulatory effects upon olfactory bulb catecholaminergic function. Therefore, events which alter estrogen levels (menstrual/estrogen cycle, pregnancy/lactation, menopause, tamoxifen treatment) can modulate olfactory bulb catecholaminergic functions which may be involved with the detection and processing of olfactory stimuli.


*Membrane Glycoproteins; *Nerve Tissue Proteins; Animals; Delayed-Action Preparations; Dopamine Plasma Membrane Transport Proteins; Drug Combinations; Estrogen Antagonists/administration & dosage; Estrogens/administration & dosage/*pharmacology; Female; Inbred Strains; Membrane Transport Proteins/*genetics/metabolism; Messenger/*metabolism; Mice; Molecular Sequence Data; Norepinephrine Plasma Membrane Transport Proteins; Olfactory Bulb/*drug effects/enzymology/*physiology; Ovariectomy; Rats; RNA; Symporters/*genetics/metabolism; Tyrosine 3-Monooxygenase/*genetics/metabolism


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Dluzen Dean E; Park June-Hee; Kim Kyungjin, “Modulation of olfactory bulb tyrosine hydroxylase and catecholamine transporter mRNA by estrogen.,” NEOMED Bibliography Database, accessed March 1, 2024,