Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

Krabill K; Jamison J M; Gilloteaux J; Summers J L

Cell biology international reports

1992

1992-10

When Mg2+ or ethidium bromide (EB) were combined with poly r(A-U) at a ligand/ribonucleotide ratio of 1/4, the antiviral activity of the Mg2+ and EB increased 136-fold and 154-fold. Eriochrome Blue SE was employed to visualize the subcellular distribution of Mg2+ following co-incubation of Human Foreskin Fibroblasts (HSF) with Mg2+ alone or with the Mg2+/poly r(A-U) combination. Phase contrast micrographs of these Mg(2+)-treated HSF cells as well as phase contrast and fluorescence micrographs of EB-treated or EB/poly r(A-U)-treated HSF cells illustrated that the Mg2+ (or EB)/poly r(A-U) combinations display altered subcellular distribution with the Mg2+ and EB being localized in the nucleoli and chromatin of the HSF cells. These results suggest that modulation of nuclear processes may be responsible for the enhanced antiviral activity.

Antiviral Agents/*pharmacology; Cells; Chromatin/drug effects; Cultured; Ethidium/*pharmacology; Humans; Magnesium/metabolism/*pharmacology; Nucleolus Organizer Region/*drug effects; Poly A-U/*pharmacology; Vesicular stomatitis Indiana virus/drug effects

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975–984

10

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