Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

Title

Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.

Creator

Krabill K; Jamison J M; Gilloteaux J; Summers J L

Publisher

Cell biology international reports

Date

1992
1992-10

Description

When Mg2+ or ethidium bromide (EB) were combined with poly r(A-U) at a ligand/ribonucleotide ratio of 1/4, the antiviral activity of the Mg2+ and EB increased 136-fold and 154-fold. Eriochrome Blue SE was employed to visualize the subcellular distribution of Mg2+ following co-incubation of Human Foreskin Fibroblasts (HSF) with Mg2+ alone or with the Mg2+/poly r(A-U) combination. Phase contrast micrographs of these Mg(2+)-treated HSF cells as well as phase contrast and fluorescence micrographs of EB-treated or EB/poly r(A-U)-treated HSF cells illustrated that the Mg2+ (or EB)/poly r(A-U) combinations display altered subcellular distribution with the Mg2+ and EB being localized in the nucleoli and chromatin of the HSF cells. These results suggest that modulation of nuclear processes may be responsible for the enhanced antiviral activity.

Subject

Antiviral Agents/*pharmacology; Cells; Chromatin/drug effects; Cultured; Ethidium/*pharmacology; Humans; Magnesium/metabolism/*pharmacology; Nucleolus Organizer Region/*drug effects; Poly A-U/*pharmacology; Vesicular stomatitis Indiana virus/drug effects

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

975–984

Issue

10

Volume

16

Citation

Krabill K; Jamison J M; Gilloteaux J; Summers J L, “Enhanced antiviral activity and altered subcellular distribution of magnesium/poly r(A-U) combinations.,” NEOMED Bibliography Database, accessed May 17, 2021, https://neomed.omeka.net/items/show/3924.

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