G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells.

G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells.

Tian Liantian; Kammermeier Paul J

Visual neuroscience

2006

2006-12

Metabotropic glutamate receptor 6 (mGluR6) is a group III, pertussis toxin (PTX)-sensitive G protein coupled mGluR that plays a specialized role in the retina. Retinal ON bipolar cells, which receive direct glutamatergic input from photoreceptor cells, express mGluR6 as their primary postsynaptic glutamate receptor. Activation of mGluR6 in these cells initiates an intracellular signaling cascade ultimately leading to inhibition of a cation channel and cell hyperpolarization. The primary mediator of this pathway in vivo is G alpha(o), but the potential roles of other G proteins from the G alpha(i/o) family in the regulation of this or other signaling pathways in ON bipolar cells are unclear. To determine which specific G proteins from the G alpha(i/o) family are able to couple to mGluR6, a G alpha reconstitution system was employed using

Animals; Calcium/metabolism; Gene Expression/drug effects/*physiology; Glutamic Acid/pharmacology; GTP-Binding Protein alpha Subunits/*metabolism; Membrane Potentials/drug effects/physiology/radiation effects; Metabotropic Glutamate/*metabolism; Microinjections/methods; Patch-Clamp Techniques/methods; Pertussis Toxin/pharmacology; Propionates/pharmacology; Rats; Receptors; Retina/*cytology; Retinal Bipolar Cells/drug effects/*metabolism/radiation effects; Reverse Transcriptase Polymerase Chain Reaction/methods; Wistar

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