Pomegranate exerts chemoprevention of experimentally induced mammary tumorigenesis by suppression of cell proliferation and induction of apoptosis.

Title

Pomegranate exerts chemoprevention of experimentally induced mammary tumorigenesis by suppression of cell proliferation and induction of apoptosis.

Creator

Bishayee Anupam; Mandal Animesh; Bhattacharyya Piyali; Bhatia Deepak

Publisher

Nutrition and cancer

Date

2016
1905-07

Description

Breast cancer is the second leading cause of cancer-related death in women in the United States and discovery and development of safe chemopreventive drugs is urgently needed. The fruit pomegranate (Punica granatum) is gaining importance because of its various health benefits. This study was initiated to investigate chemopreventive potential of a pomegranate emulsion (PE) against 7,12-dimethylbenz(a)anthracene (DMBA) rat mammary carcinogenesis. The animals were orally administered with PE (0.2-5.0 g/kg), starting 2 wk before and 16 wk following DMBA treatment. PE exhibited a striking reduction of DMBA-induced mammary tumor incidence, total tumor burden, and reversed histopathological changes. PE dose-dependently suppressed cell proliferation and induced apoptosis in mammary tumors. Immunohistochemical studies showed that PE increased intratumor Bax, decreased Bcl2 and manifested a proapoptotic shift in Bax/Bcl2 ratio. In addition, our gene expression study showed PE-mediated upregulation of Bad, caspase-3, caspase-7, caspase-9, poly (ADP ribose) polymerase and cytochrome c in mammary tumors. Thus, PE exerts chemoprevention of mammary carcinogenesis by suppressing cell proliferation and inducing apoptosis mediated through upregulation of Bax and downregulation of Bcl2 in concert with caspase cascades. Pomegranate bioactive phytoconstituents could be developed as a chemopreventive drug to reduce the risk of breast cancer.

Subject

*Punicaceae/chemistry; 10-Dimethyl-1; 2-benzanthracene; 9; Animal Studies; Animals; Anticarcinogenic Agents/*therapeutic use; Apoptosis; Apoptosis/*drug effects; Breast Neoplasms – Mortality; Breast Neoplasms – Prevention and Control; Breast Neoplasms – Risk Factors; Cell Physiology; Cell Proliferation/*drug effects; Diet – Evaluation; Experimental/pathology/*prevention & control; Female; Funding Source; Gene Expression Regulation; Genes; Human; Immunohistochemistry; Mammary Neoplasms; Mutation; National Institutes of Health (U.S.); Neoplasms – Prevention and Control; Phytotherapy; Pomegranate; Proto-Oncogene Proteins c-bcl-2/analysis; Rats; Sprague-Dawley; Tamoxifen; United States

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

120–130

Issue

1

Volume

68

Citation

Bishayee Anupam; Mandal Animesh; Bhattacharyya Piyali; Bhatia Deepak, “Pomegranate exerts chemoprevention of experimentally induced mammary tumorigenesis by suppression of cell proliferation and induction of apoptosis.,” NEOMED Bibliography Database, accessed June 18, 2021, https://neomed.omeka.net/items/show/4092.

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