Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present.

Title

Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present.

Creator

Rothschild B M; Martin L D; Lev G; Bercovier H; Bar-Gal G K; Greenblatt C; Donoghue H; Spigelman M; Brittain D

Publisher

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

Date

2001
2001-08

Description

In order to assess the presence of tuberculosis in Pleistocene bison and the origin of tuberculosis in North America, 2 separate DNA extractions were performed by 2 separate laboratories on samples from the metacarpal of an extinct long-horned bison that was radiocarbon dated at 17,870+/-230 years before present and that had pathological changes suggestive of tuberculosis. Polymerase chain reaction amplification isolated fragments of tuberculosis DNA, which were sequenced, and on which spoligotyping was also performed to help determine its relationship to the various members of the Mycobacterium tuberculosis complex. Extensive precautions against contamination with modern M. tuberculosis complex DNA were employed, including analysis of paleontologic and modern specimens in 2 geographically separate laboratories.

Subject

Ancient; Animals; Bacterial/*analysis/history; Bison/*microbiology; DNA; History; Mycobacterium tuberculosis/*genetics; Paleontology; Tuberculosis/*history/veterinary; Wyoming

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

305–311

Issue

3

Volume

33

Citation

Rothschild B M; Martin L D; Lev G; Bercovier H; Bar-Gal G K; Greenblatt C; Donoghue H; Spigelman M; Brittain D, “Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present.,” NEOMED Bibliography Database, accessed October 27, 2021, https://neomed.omeka.net/items/show/4138.

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