Monitoring the synthesis of biomolecules using mass spectrometry.

Title

Monitoring the synthesis of biomolecules using mass spectrometry.

Creator

Miyagi Masaru; Kasumov Takhar

Publisher

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences

Date

2016
2016-10

Description

The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or metabolites). It is, however, important to recognize that biological systems are highly dynamic in which biomolecules are continuously renewed and different classes of biomolecules interact and affect each other's production/clearance. Therefore, it is necessary to measure the turnover of diverse classes of biomolecules to understand the dynamic nature of biological systems. Herein, we explain why the kinetic analysis of a diverse range of biomolecules is important and how such an analysis can be done. We argue that heavy water ((2)H2O) could be a universal tracer for monitoring the synthesis of biomolecules on a global scale.This article is part of the themed issue 'Quantitative mass spectrometry'.

Subject

biosynthesis; Deuterium Oxide/chemistry; deuterium water; flux analysis; Kinetics; Macromolecular Substances/*chemistry/*metabolism; mass spectrometry; Mass Spectrometry/*methods; metabolomics; proteomics

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Issue

2079

Volume

374

Citation

Miyagi Masaru; Kasumov Takhar, “Monitoring the synthesis of biomolecules using mass spectrometry.,” NEOMED Bibliography Database, accessed January 25, 2021, https://neomed.omeka.net/items/show/4432.

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