Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

Ganguly Rituparna; Sahu Soumyadip; Chavez Ronaldo J; Raman Priya

American journal of physiology. Cell physiology

2015

2015-01

Trivalent chromium (Cr(3+)) is a mineral nutrient reported to have beneficial effects in glycemic and cardiovascular health. In vitro and in vivo studies suggest that Cr(3+) supplementation reduces the atherogenic potential and lowers the risk of vascular inflammation in diabetes. However, effects of Cr(3+) in vascular cells under conditions of hyperglycemia, characteristic of diabetes, remain unknown. In the present study we show that a therapeutically relevant concentration of Cr(3+) (100 nM) significantly downregulates a potent proatherogenic matricellular protein, thrombospondin-1 (TSP-1), in human aortic smooth muscle cells (HASMC) stimulated with high glucose in vitro. Promoter-reporter assays reveal that this downregulation of TSP-1 expression by Cr(3+) occurs at the level of transcription. The inhibitory effects of Cr(3+) on

Aorta/drug effects/metabolism; Cell Proliferation/*drug effects/genetics; Cells; Chromium/*pharmacology; Cultured; Fructosephosphates/metabolism; Genetic/drug effects/genetics; Glucose/*metabolism; Glutamine/genetics; Glycosylation/drug effects; Hexosamines/metabolism; Humans; Hyperglycemia/metabolism; Muscle; Myocytes; N-Acetylglucosaminyltransferases/genetics; O-glycosylation; Promoter Regions; reactive oxygen species; Reactive Oxygen Species/metabolism; Signal Transduction/*drug effects/genetics; Smooth; Smooth Muscle/*drug effects/metabolism; Thrombospondin 1/*antagonists & inhibitors/genetics; thrombospondin-1; Transcription; trivalent chromium; vascular smooth muscle cells; Vascular/*drug effects/metabolism

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