Congenital diaphragmatic hernia prevents absorption of distal air space fluid in late-gestation rat fetuses.

Congenital diaphragmatic hernia prevents absorption of distal air space fluid in late-gestation rat fetuses.

Folkesson Hans G; Chapin Cheryl J; Beard LaMonta L; Ertsey Robert; Matthay Michael A; Kitterman Joseph A

American journal of physiology. Lung cellular and molecular physiology

2006

2006-03

We hypothesized that congenital diaphragmatic hernia (CDH) may decrease distal air space fluid absorption due to immaturity of alveolar epithelial cells from a loss of the normal epithelial Na+ transport, as assessed by amiloride and epithelial Na+ channel (ENaC) and Na-K-ATPase expression, as well as failure to respond to endogenous epinephrine as assessed by propranolol. Timed-pregnant dams were gavage fed 100 mg of nitrofen at 9.5-day gestation to induce CDH in the fetuses, and distal air space fluid absorption experiments were carried out on

*Gestational Age; *Hernias; Absorption; Amiloride/pharmacology; Animals; Body Fluids/*metabolism; Congenital; Diaphragmatic; Diaphragmatic/*metabolism; Epinephrine/metabolism; Epithelial Sodium Channels; Female; Fetal Organ Maturity; Fetus/*metabolism; Hernia; Male; Newborn; Phenyl Ethers/administration & dosage; Pregnancy; Propranolol/pharmacology; Rats; Respiratory System Abnormalities; Sodium Channels/metabolism; Sodium-Potassium-Exchanging ATPase/metabolism; Sprague-Dawley

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

L478–484

3

290