Effect of high transcapillary pressures on capillary ultrastructure and permeability coefficients in dog lung.

Title

Effect of high transcapillary pressures on capillary ultrastructure and permeability coefficients in dog lung.

Creator

Maron M B; Fu Z; Mathieu-Costello O; West J B

Publisher

Journal of applied physiology (Bethesda, Md. : 1985)

Date

2001
2001-02

Description

To determine the correlation between ultrastructural and physiological changes in blood-gas barrier function in lungs transiently exposed to very high vascular pressures, we increased capillary transmural pressure (Ptm) of 6 canine isolated perfused left lower lung lobe preparations (high-pressure group) to 80.3 Torr for 3.8 min and then determined the capillary filtration (K(fc)) and osmotic reflection (sigma(d)) coefficients at a Ptm of 19.1 Torr in the ventilated lung lobes. This was followed by perfusion fixation of the lobes at a Ptm of 20.5 Torr for ultrastructural analysis. These data were compared with those obtained in six lobes in which Ptm was not transiently elevated before K(fc), sigma(d), and ultrastructural evaluation. K(fc) was higher [0.249 +/- 0.042 (SE) vs. 0.054 +/- 0.009 g. min(-1). Torr(-1). 100 g(-1); P \textless 0.01] and sigma(d) was lower (0.52 +/- 0.07 vs. 0.85 +/- 0.08; P \textless 0.01) in the high-pressure group. In contrast, although endothelial and epithelial breaks were occasionally observed in some experiments, their incidence was not increased in the high-pressure group. These data suggest that the increased transvascular water and protein flux occurred through pathways of a size not resolvable by electron microscopy after vascular perfusion-fixation at a Ptm of 20.5 Torr.

Subject

*Blood-Air Barrier; *Pulmonary Circulation; Animals; Blood Pressure; Capillaries/cytology/*physiology/*ultrastructure; Dogs; Electron; Endothelium; Female; Lung/blood supply/*ultrastructure; Male; Microscopy; Non-programmatic; Osmotic Pressure; Respiratory Mucosa/ultrastructure; Vascular/ultrastructure

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

638–648

Issue

2

Volume

90

Citation

Maron M B; Fu Z; Mathieu-Costello O; West J B, “Effect of high transcapillary pressures on capillary ultrastructure and permeability coefficients in dog lung.,” NEOMED Bibliography Database, accessed August 3, 2021, https://neomed.omeka.net/items/show/4660.

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