Tissue engineering models of human digits: effect of periosteum on growth plate cartilage development.

Title

Tissue engineering models of human digits: effect of periosteum on growth plate cartilage development.

Creator

Landis William J; Jacquet Robin; Lowder Elizabeth; Enjo Mitsuhiro; Wada Yoshitaka; Isogai Noritaka

Publisher

Cells, tissues, organs

Date

2009
2009

Description

Tissue-engineered middle phalanx constructs of human digits were investigated to determine whether periosteum wrapped partly about model midshafts mediated cartilage growth plate formation. Models were fabricated by suturing ends of polymer midshafts in a human middle phalanx shape with polymer sheets seeded with heterogeneous chondrocyte populations from bovine articular cartilage. Half of each midshaft length was wrapped with bovine periosteum. Constructs were cultured, implanted in nude mice for up to 20 weeks, harvested and treated histologically to assess morphology and cartilage proteoglycans. After 20 weeks of implantation, chondrocyte-seeded sheets adjacent to periosteum-wrapped midshaft halves established cartilage growth plates resembling normal tissue in vivo. Sheets adjacent to midshafts without periosteum had disorganized cells and no plate formation. Proteoglycans were present at both midshaft ends. Periosteum appears to guide chondrocytes toward growth plate cartilage organization and tissue engineering provides means for carefully examining construct development of this tissue.

Subject

*Models; *Tissue Engineering; Animals; Articular/*growth & development; Biological; Cartilage; Cattle; Experimental; Fingers/diagnostic imaging; Growth Plate/cytology/*growth & development; Humans; Implants; Male; Mice; Nude; Periosteum/*physiology; Radiography

Identifier

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

241–244

Issue

1

Volume

189

Citation

Landis William J; Jacquet Robin; Lowder Elizabeth; Enjo Mitsuhiro; Wada Yoshitaka; Isogai Noritaka, “Tissue engineering models of human digits: effect of periosteum on growth plate cartilage development.,” NEOMED Bibliography Database, accessed January 25, 2022, https://neomed.omeka.net/items/show/4727.

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