Interleukin-1/toll-like receptor-induced nuclear factor kappa B signaling participates in intima hyperplasia after carotid artery balloon injury in goto-kakizaki rats: a potential target therapy pathway.

Title

Interleukin-1/toll-like receptor-induced nuclear factor kappa B signaling participates in intima hyperplasia after carotid artery balloon injury in goto-kakizaki rats: a potential target therapy pathway.

Creator

Zhang Xiaotian; Wang Yi; Hu Wenjing; Li Dongye; Zhou Zhongmin; Pan Defeng; Wu Wanling; Xu Tongda

Publisher

PloS one

Date

2014
2014

Description

The value of restenosis after percutaneous coronary intervention (PCI) is recognized worldwide, especially for diabetic patients. Interleukin-1/Toll-like receptor (IL-1/TLR) signaling is involved in innate and adaptive immune responses, but whether and how the IL-1/TLR-induced nuclear factor kappa B (NFkappaB) pathway plays key roles in intimal formation is unclear. The underlying mechanism of intima hyperplasia was investigated with a model of carotid balloon injury in Goto-Kakizaki (GK) and Wistar rats and with lipopolysaccharide-stimulated macrophages. Elastic-van Gieson staining showed the medial area peakedon Day 3 post-injury and decreased by Day 7 post-injury in both GK and Wistar rats. The N/M at Day 7 in GK rats was significantly higher than in Wistar rats (p\textless0.001). The percent of 5-ethynyl-2'-deoxyuridine (EdU) staining-positive cells on Day 3 post-injury was greater than seen on Day 7 post-injury in GK and Wistar rats. The percent of EdU-positive cells on Days 3 and 7 post-injury in Wistar rats was less than that found in GK rats (p\textless0.01; p\textless0.05). NFkappaBp65 immunostaining had increased by Day 7 post-injury. Agilent Whole Genome Oligo Microarray verified that the IL-1/TLR-induced NFkappaB pathway was activated by carotid balloon injury. TLR4, IL-1 receptor associated kinase, inhibitors alpha of NFkappaB, human antigen R, c-Myc (Proto-Oncogene Proteins),

Subject

Male; Animals; Rats; NF-kappa B/*metabolism; Signal Transduction/genetics/physiology; Carotid Artery Injuries/*metabolism; Hyperplasia/*metabolism; Interleukin-1/*metabolism; Toll-Like Receptors/*metabolism; Tunica Intima/*metabolism/*pathology; Wistar

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

e103794–e103794

Issue

8

Volume

9

Citation

Zhang Xiaotian; Wang Yi; Hu Wenjing; Li Dongye; Zhou Zhongmin; Pan Defeng; Wu Wanling; Xu Tongda, “Interleukin-1/toll-like receptor-induced nuclear factor kappa B signaling participates in intima hyperplasia after carotid artery balloon injury in goto-kakizaki rats: a potential target therapy pathway.,” NEOMED Bibliography Database, accessed April 21, 2021, https://neomed.omeka.net/items/show/4948.

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