Gelucire-stabilized nanoparticles as a potential DNA delivery system.

Gelucire-stabilized nanoparticles as a potential DNA delivery system.

Oyewumi Moses O; Wehrung Daniel; Sadana Prabodh

Pharmaceutical development and technology

2016

2016-09

Clinical viability of gene delivery systems has been greatly impacted by potential toxicity of the delivery systems. Recently, we reported the nanoparticle (NP) preparation process that employs biocompatible materials such as Gelucire(R) 44/14 and cetyl alcohol as matrix materials. In the current study, the NP preparation was modified for pDNA loading through: (i) inclusion of cationic lipids (DOTAP or DDAB) with NP matrix materials; or (ii) application of cationic surfactants (CTAB) to generate NPs with desired surface charges for pDNA complexation. Colloidal stability and efficiency of loading pGL3-DR4X2-luciferase plasmid DNA in NPs were verified by gel permeation chromatography. Compared to pDNA alone, all the NPs were effective in preserving pDNA from digestion by DNase. While pDNA loading using CTAB-NPs involved fewer steps compared to

Humans; Animals; Mice; nanoparticles; Cell Line; Hep G2 Cells; gene therapy; *Gene Transfer Techniques; Cationic lipids; Cell Survival/drug effects/physiology; DNA/*administration & dosage/chemistry; macrophage activation; Macrophages/drug effects/physiology; Nanoparticles/*administration & dosage/chemistry; Polyethylene Glycols/*administration & dosage/chemistry; transfection

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647–654

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