Gelucire-stabilized nanoparticles as a potential DNA delivery system.

Title

Gelucire-stabilized nanoparticles as a potential DNA delivery system.

Creator

Oyewumi Moses O; Wehrung Daniel; Sadana Prabodh

Publisher

Pharmaceutical development and technology

Date

2016
2016-09

Description

Clinical viability of gene delivery systems has been greatly impacted by potential toxicity of the delivery systems. Recently, we reported the nanoparticle (NP) preparation process that employs biocompatible materials such as Gelucire(R) 44/14 and cetyl alcohol as matrix materials. In the current study, the NP preparation was modified for pDNA loading through: (i) inclusion of cationic lipids (DOTAP or DDAB) with NP matrix materials; or (ii) application of cationic surfactants (CTAB) to generate NPs with desired surface charges for pDNA complexation. Colloidal stability and efficiency of loading pGL3-DR4X2-luciferase plasmid DNA in NPs were verified by gel permeation chromatography. Compared to pDNA alone, all the NPs were effective in preserving pDNA from digestion by DNase. While pDNA loading using CTAB-NPs involved fewer steps compared to

Subject

Humans; Animals; Mice; nanoparticles; Cell Line; Hep G2 Cells; gene therapy; *Gene Transfer Techniques; Cationic lipids; Cell Survival/drug effects/physiology; DNA/*administration & dosage/chemistry; macrophage activation; Macrophages/drug effects/physiology; Nanoparticles/*administration & dosage/chemistry; Polyethylene Glycols/*administration & dosage/chemistry; transfection

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

647–654

Issue

6

Volume

21

Citation

Oyewumi Moses O; Wehrung Daniel; Sadana Prabodh, “Gelucire-stabilized nanoparticles as a potential DNA delivery system.,” NEOMED Bibliography Database, accessed January 20, 2021, https://neomed.omeka.net/items/show/5108.

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