Absolute CD4 counts obtained by a three-color flow-cytometric method without the use of a hematology analyzer.

Absolute CD4 counts obtained by a three-color flow-cytometric method without the use of a hematology analyzer.

Alexander T S

Clinical and diagnostic laboratory immunology

1998

1998-03

We evaluated the Ortho TRIO-Cytoronabsolute system for determining absolute CD4 counts. The CD4 counts in our blood specimens from 100 individuals ranged from 3 to 1,962; the percent CD4 ranged from 1.3 to 62.2, respectively. The TRIO system was biased toward lower absolute counts than a combination of flow cytometry and hematology but showed no bias in percent CD4 calculations.

Humans; CD4-Positive T-Lymphocytes/*pathology; Flow Cytometry/*methods; Lymphocyte Count/*methods

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266–269

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