Analysis of fluorescently labeled substance P analogs: binding, imaging and receptor activation.

Title

Analysis of fluorescently labeled substance P analogs: binding, imaging and receptor activation.

Creator

Bennett Vicki J; Simmons Mark A

Publisher

BMC chemical biology

Date

2001
1905-06

Description

BACKGROUND: Substance P (SP) is a peptide neurotransmitter found in central and peripheral nerves. SP is involved in the control of smooth muscle, inflammation and nociception. The amino acid sequence of SP is Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2. Five different forms of fluorescently labeled SP have recently been synthesized, in which Alexa 488, BODIPY Fl, fluorescein, Oregon Green 488 or tetramethylrhodamine has been covalently linked to SP at Lys3. Here, these novel analogs are characterized as to their ligand binding, receptor activation and fluorescence labeling properties. RESULTS: Competition binding studies, using radiolabeled [125I] SP, revealed that all of the labeled forms of SP, except for Alexa 488-SP, effectively competed with radiolabeled SP for binding at the rat SP receptor. With the exception of Alexa 488-SP, all of the SP analogs produced Ca++ elevations and fluorescence labeling of the SP receptor expressed in Chinese hamster ovary cells. In SP-responsive neurons, BODIPY Fl-SP and Oregon Green

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1–1

Issue

1

Volume

1

Citation

Bennett Vicki J; Simmons Mark A, “Analysis of fluorescently labeled substance P analogs: binding, imaging and receptor activation.,” NEOMED Bibliography Database, accessed May 9, 2021, https://neomed.omeka.net/items/show/5308.

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