A slice preparation preserving the callosal projection to contralateral visual cortex.

Title

A slice preparation preserving the callosal projection to contralateral visual cortex.

Creator

Berry R L; Nowicky A; Teyler T J

Publisher

Journal of neuroscience methods

Date

1990
1990-08

Description

Due to the curved path they follow, the visual callosal projections to areas OC1 and OC2 of the rat visual cortex have been inaccessible to studies using brain slices. In this paper we describe a new slice preparation in which a curved cutting blade was used to obtain slices in which callosal fibers projecting to OC1 or OC2 are preserved. Stimulation of the contralateral white matter resulted in EPSPs recorded in layer II/III and V cells of OC2 studied with intracellular recording. Current source density analysis of extracellular field potentials collected in OC1 and OC2 revealed laminar current sink patterns paralleling the laminar distribution of callosal terminations reported by Miller and Vogt (Dev. Brain Res., 14 (1984) 304-309). Exposure of slices to 2 mM kynurenic acid reversibly abolished current sinks in OC1 recorded in response to callosal stimulation indicating that glutamate receptors mediate the response of OC1 to callosal afferent activity. This new slicing technique can be readily adapted to study other systems in the nervous system in which neural processes follow curved trajectories.

Subject

Female; Male; Animals; Rats; *Synaptic Transmission; In Vitro Techniques; Electrophysiology; Corpus Callosum/*physiology; Neurology/instrumentation; Visual Cortex/*physiology; Inbred Strains

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

171–178

Issue

2

Volume

33

Citation

Berry R L; Nowicky A; Teyler T J, “A slice preparation preserving the callosal projection to contralateral visual cortex.,” NEOMED Bibliography Database, accessed December 2, 2021, https://neomed.omeka.net/items/show/5312.

Social Bookmarking