Immunocytochemical analysis of somatostatin in the hypothalamus of obese and non-obese Zucker rats.

Title

Immunocytochemical analysis of somatostatin in the hypothalamus of obese and non-obese Zucker rats.

Creator

DonCarlos L L; Ho R H; Finkelstein J A

Publisher

Brain research

Date

1988
1988-08

Description

Levels of growth hormone (GH) are reduced in the genetically obese Zucker rat, fa/fa, in comparison to lean littermates. In normal rats, GH release is regulated by stimulatory and inhibitory factors of hypothalamic origin. The present experiment focuses on hypothalamic somatostatin (SOM; growth hormone release inhibiting factor) in order to determine if abnormal hypothalamic SOM may be a correlate of depressed GH secretion in fa/fa rats. We compared immunocytochemical localization of hypothalamic SOM between 5 obese (fa/fa) Zucker rats and 5 non-obese littermates. Brain sections from pairs of animals were processed simultaneously. The distribution of SOM immunoreactive cell bodies in the hypothalamus agreed with previous reports. SOM-containing neurons in the periventricular area were counted and analyzed at 4 hypothalamic levels: (1) anterior to the suprachiasmatic nucleus (SCN); (2) through SCN; (3) between SCN and the ventromedial hypothalamic nucleus (VMH); and (4) through VMH. The greatest number of SOM-immunoreactive cell bodies was observed at levels (2) and (3). The numbers of SOM-containing cells did not differ significantly between obese and lean animals. No apparent difference in density of fiber staining was observed in the median eminence.

Subject

Animals; Rats; Species Specificity; Obesity/*metabolism; Immunoenzyme Techniques; Hypothalamus/*cytology/pathology; Somatostatin/*analysis/immunology; Zucker

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

372–376

Issue

2

Volume

458

Citation

DonCarlos L L; Ho R H; Finkelstein J A, “Immunocytochemical analysis of somatostatin in the hypothalamus of obese and non-obese Zucker rats.,” NEOMED Bibliography Database, accessed July 31, 2021, https://neomed.omeka.net/items/show/5382.

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