An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.
Title
An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.
Creator
Donzanti B A; Yamamoto B K
Publisher
Life sciences
Date
1988
1905-06
Description
An improved, HPLC with electrochemical detection method for the isocratic separation and determination of amino acids from post-mortem brain tissue and from microdialysates of awake-behaving animals is described. Optimal conditions that maximize stability, resolution, and sensitivity were determined for the pre-column derivatization of amino acids using o-phthalaldehyde and
Subject
Dialysis; Male; Animals; Rats; *Brain Chemistry; Chromatography; Electrochemistry; Corpus Striatum/analysis; Amino Acids/*analysis; Neurotransmitter Agents/*analysis; Inbred Strains; High Pressure Liquid/*methods
Identifier
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Citation
Donzanti B A; Yamamoto B K, “An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.,” NEOMED Bibliography Database, accessed April 19, 2024, https://neomed.omeka.net/items/show/5384.