An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.

Title

An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.

Creator

Donzanti B A; Yamamoto B K

Publisher

Life sciences

Date

1988
1905-06

Description

An improved, HPLC with electrochemical detection method for the isocratic separation and determination of amino acids from post-mortem brain tissue and from microdialysates of awake-behaving animals is described. Optimal conditions that maximize stability, resolution, and sensitivity were determined for the pre-column derivatization of amino acids using o-phthalaldehyde and

Subject

Dialysis; Male; Animals; Rats; *Brain Chemistry; Chromatography; Electrochemistry; Corpus Striatum/analysis; Amino Acids/*analysis; Neurotransmitter Agents/*analysis; Inbred Strains; High Pressure Liquid/*methods

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

913–922

Issue

11

Volume

43

Citation

Donzanti B A; Yamamoto B K, “An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.,” NEOMED Bibliography Database, accessed October 25, 2021, https://neomed.omeka.net/items/show/5384.

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