Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Karam W G; Chiang J Y

Journal of lipid research

1994

1994-07

Cholesterol 7 alpha-hydroxylase (P450c7) is the first and rate-limiting enzyme in bile acid biosynthesis and is the product of a cytochrome P450 gene, CYP7. We have previously reported the cloning of a full-length human cholesterol 7 alpha-hydroxylase cDNA (Karam, W. G., and J. Y. L. Chiang. 1992. Biochem. Biophys. Res. Commun. 185: 588-595). Using this clone in a polymerase chain reaction, we have generated a cDNA (H7 alpha 1.5) in which the codons for the

Humans; Animals; Rats; Kinetics; Base Sequence; Catalysis; Molecular Sequence Data; Escherichia coli; Solubility; Cholesterol 7-alpha-Hydroxylase/biosynthesis/genetics/*isolation & purification; Cytochrome P-450 Enzyme System/biosynthesis/isolation & purification; Immunochemistry; Recombinant Proteins/biosynthesis; Subcellular Fractions/enzymology

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1222–1231

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