Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Title

Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.

Creator

Karam W G; Chiang J Y

Publisher

Journal of lipid research

Date

1994
1994-07

Description

Cholesterol 7 alpha-hydroxylase (P450c7) is the first and rate-limiting enzyme in bile acid biosynthesis and is the product of a cytochrome P450 gene, CYP7. We have previously reported the cloning of a full-length human cholesterol 7 alpha-hydroxylase cDNA (Karam, W. G., and J. Y. L. Chiang. 1992. Biochem. Biophys. Res. Commun. 185: 588-595). Using this clone in a polymerase chain reaction, we have generated a cDNA (H7 alpha 1.5) in which the codons for the

Subject

Humans; Animals; Rats; Kinetics; Base Sequence; Catalysis; Molecular Sequence Data; Escherichia coli; Solubility; Cholesterol 7-alpha-Hydroxylase/biosynthesis/genetics/*isolation & purification; Cytochrome P-450 Enzyme System/biosynthesis/isolation & purification; Immunochemistry; Recombinant Proteins/biosynthesis; Subcellular Fractions/enzymology

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1222–1231

Issue

7

Volume

35

Citation

Karam W G; Chiang J Y, “Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.,” NEOMED Bibliography Database, accessed April 25, 2024, https://neomed.omeka.net/items/show/5472.