Polymorphisms of human cholesterol 7 alpha-hydroxylase.
Title
Polymorphisms of human cholesterol 7 alpha-hydroxylase.
Creator
Karam W G; Chiang J Y
Publisher
Biochemical and biophysical research communications
Date
1992
1992-06
Description
Human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs were isolated from a human liver cDNA library. A full-length cDNA has 2901 nucleotides which encode a typical P450 polypeptide of 504 amino acid residues. Two different sequences of codon 100, TTT (Phe) and TCT (Ser), were identified in cDNA clones. In addition, codons 347 and 385 are GAT (Asp) and GAC (Asp) in all cDNA clones, whereas those reported previously (FEBS Lett. 268, 137-140, 1990) are AAT (Asn) and AGC (Ser), respectively. Since there is only one 7 alpha-hydroxylase gene in the human genome, it is likely that polymorphisms at the codon 100 of cDNA clones arise from two different alleles in the 7 alpha-hydroxylase gene of this human liver.
Subject
Humans; Amino Acid Sequence; Base Sequence; Polymerase Chain Reaction; Molecular Sequence Data; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA/genetics; Restriction Mapping; Oligodeoxyribonucleotides/chemistry; Genetic; Cloning; Molecular; Polymorphism
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
588–595
Issue
2
Volume
185
Citation
Karam W G; Chiang J Y, “Polymorphisms of human cholesterol 7 alpha-hydroxylase.,” NEOMED Bibliography Database, accessed April 19, 2024, https://neomed.omeka.net/items/show/5473.