Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.

Title

Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.

Creator

Lai T S; Chiang J Y

Publisher

Archives of biochemistry and biophysics

Date

1990
1990-12

Description

We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two P450s were present in very low levels in control hamster livers and were greatly induced by 3-methylcholanthrene treatment. P450 MC1 was immunochemically different from P450 MC4, rat P450c and P450d, and rabbit LM4. The immunorelated polypeptide to P450 MC1 was not present in the control or the

Subject

Female; Humans; Male; Animals; Rats; Amino Acid Sequence; Reference Values; Base Sequence; Liver/enzymology; Immunoblotting; Molecular Sequence Data; Cricetinae; Mesocricetus; Phenobarbital/pharmacology; Restriction Mapping; Nucleic Acid Hybridization; Cytochrome P-450 Enzyme System/biosynthesis/*genetics; Enzyme Induction; Liver/drug effects/*enzymology; Methylcholanthrene/*pharmacology; Inbred Strains; Sequence Homology; Cloning; Molecular; Nucleic Acid; Microsomes

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

429–439

Issue

2

Volume

283

Citation

Lai T S; Chiang J Y, “Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.,” NEOMED Bibliography Database, accessed March 6, 2021, https://neomed.omeka.net/items/show/5497.

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