Baclofen-induced disinhibition in area CA1 of rat hippocampus is resistant to extracellular Ba2+.

Baclofen-induced disinhibition in area CA1 of rat hippocampus is resistant to extracellular Ba2+.

Lambert N A; Harrison N L; Teyler T J

Brain research

1991

1991-05

The mechanism of disinhibition produced by (+/-)-baclofen was studied using intracellular recording in area CA1 of rat hippocampal slices. Baclofen reversibly depressed monosynaptic IPSPs evoked by direct activation of interneurons in the presence of the excitatory amino acid receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and D,L-2-amino-5-phosphonovalerate (APV). Ba2+ prevented baclofen-induced hyperpolarization of pyramidal neurons but not depression of monosynaptic IPSPs by baclofen. Baclofen reversibly depressed monosynaptic IPSPs when applied close to the recording site, but was ineffective when applied close to the stimulating site in stratum radiatum. These results suggest that baclofen disinhibits pyramidal neurons in area CA1 of the rat hippocampus by activating receptors on the terminals of inhibitory neurons that are coupled to a Ba(2+)-insensitive effector mechanism.

Animals; Rats; In Vitro Techniques; Quinoxalines/pharmacology; 2-Amino-5-phosphonovalerate/pharmacology; Hippocampus/*drug effects; Evoked Potentials/drug effects; Baclofen/antagonists & inhibitors/*pharmacology; Barium/*pharmacology; Drug Resistance/physiology; Interneurons/drug effects; Neural Inhibition/drug effects; Receptors; GABA-A/*drug effects

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