Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines.

Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines.

Phonphok Y; Rosenthal K S

FEBS letters

1991

1991-04

Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25 degrees C or 37 degrees C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride (10 mM), chloroquine (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.

Animals; Swine; Structure-Activity Relationship; Kinetics; Amantadine/*analogs & derivatives/*pharmacology; Amines/*pharmacology; Brain/metabolism; Clathrin/*metabolism; Cell-Membrane/drug effects/*metabolism; Coated Pits

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