Herpes simplex virus type 1 penetration initiates mobilization of cell surface proteins.

Title

Herpes simplex virus type 1 penetration initiates mobilization of cell surface proteins.

Creator

Rosenthal K S; Roess D; Barisas B G

Publisher

Biochimica et biophysica acta

Date

1988
1988-07

Description

Changes in membrane structure resulting from herpes simplex virus 1 (HSV-1) penetration were detected using fluorescence photobleaching recovery methods. The effect could be blocked by inhibitors of viral and cellular processes involved in virus penetration. A rapid mode of HSV-1 strain KOS penetration into VERO cells at 37 degrees C normally occurs after a 5 min lag period and is 90-95% complete within 20-30 min. Rates of cell surface protein diffusion increase 2-3-fold after 5 min and return to normal after 25-30 min, this return correlating temporally with the penetration of the virus. At pH 6.3 the lag period preceeding penetration of HSV is increased to 20 min and penetration proceeds much more slowly than at pH 7.4. Inhibition of virus penetration with cytochalasin B or with the antiherpes drug tromantadine also prevents the HSV-1-induced increase in cell surface protein mobility. Colchicine, which does not block HSV-1 penetration, prevents the recovery of the membrane following virus penetration. Therefore, the changes in membrane structure characterized by increased cell surface protein mobility seem to be caused by virus penetration. Cytoskeletal function and integrity are required for the initiation of, and cell recovery from, virus penetration. A pH-sensitive activity, likely to be a virion fusion glycoprotein, is also required.

Subject

Animals; Hydrogen-Ion Concentration; In Vitro Techniques; Actin Cytoskeleton/physiology; Vero Cells; Microscopy; Endocytosis; Amantadine/analogs & derivatives/pharmacology; Colchicine/pharmacology; Cytochalasin B/pharmacology; Herpes Simplex/*physiopathology; Membrane Fluidity; Membrane Proteins/*physiology; Microtubules/physiology; Simplexvirus/*physiology; Fluorescence

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

38–44

Issue

1

Volume

942

Citation

Rosenthal K S; Roess D; Barisas B G, “Herpes simplex virus type 1 penetration initiates mobilization of cell surface proteins.,” NEOMED Bibliography Database, accessed May 13, 2021, https://neomed.omeka.net/items/show/5588.

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