Characterization of a homogeneous paraprotein from a horse with spontaneous multiple myeloma syndrome.

Title

Characterization of a homogeneous paraprotein from a horse with spontaneous multiple myeloma syndrome.

Creator

Seide R K; Jacobs R M; Dobblestein T N; Kehoe J M

Publisher

Veterinary immunology and immunopathology

Date

1987
1987-12

Description

A novel myeloma paraprotein has been isolated from a horse with a lymphoid tumor. The protein was a euglobulin and consequently was readily isolated from serum in pure form and high yield by simple dilution in distilled water. The purified intact protein had a molecular weight of 150,000 and was composed of heavy and light chains, both of which had blocked amino-termini and were thus not susceptible to amino-terminal sequence analysis. The amino acid compositions of these respective chains corresponded to those of comparable chains from immunoglobulins of other species. Peptide maps of paraprotein light chains prepared by high pressure liquid chromatography corresponded in part to those of normal pooled equine light chains. The identification of this paraprotein as an equine AI (aggregating immunoglobulin) protein was confirmed by serological analysis using a specific antiserum. The relationship of this particular protein to other members of the immunoglobulin family was further demonstrated by the production of an anti-idiotypic antiserum individually specific for this molecule.

Subject

Male; Animals; Horse Diseases/*immunology; Horses/immunology; Immunoglobulin Light Chains/*isolation & purification; Multiple Myeloma/immunology/*veterinary; Myeloma Proteins/*isolation & purification

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

69–77

Issue

1

Volume

17

Citation

Seide R K; Jacobs R M; Dobblestein T N; Kehoe J M, “Characterization of a homogeneous paraprotein from a horse with spontaneous multiple myeloma syndrome.,” NEOMED Bibliography Database, accessed January 24, 2022, https://neomed.omeka.net/items/show/5636.

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