The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.

Title

The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.

Creator

Yoo M; Steggles A W

Publisher

Biochemical and biophysical research communications

Date

1989
1989-08

Description

We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A) addition signal. In addition, all the clones contained the coding information for amino acids 87-134; however, there were two types of intron junction adjacent to the coding sequence. Detailed analysis of the Type I clones showed that the Type II intron sequence was contained within the Type I sequence, but approximately 1000 bp 5' of the Type I intron-exon junction showed alternative splicing within this intron. In addition, we have isolated two pseudogenes which lack introns, suggesting the retroviral insertion of human liver cytochrome b5 mRNA sequences into the human genome.

Subject

Humans; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Liver/*physiology; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; Pseudogenes; Cloning; Molecular; Post-Transcriptional; RNA Processing

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

18–24

Issue

1

Volume

163

Citation

Yoo M; Steggles A W, “The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.,” NEOMED Bibliography Database, accessed March 4, 2021, https://neomed.omeka.net/items/show/5718.

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