Contribution of amiloride-insensitive pathways to alveolar fluid clearance in adult rats

Title

Contribution of amiloride-insensitive pathways to alveolar fluid clearance in adult rats

Creator

Norlin A; Lu L N; Guggino S E; Matthay M A; Folkesson H G

Publisher

Journal of Applied Physiology

Date

2001
2001-04

Description

The contributions of amiloride-sensitive and -insensitive fractions of alveolar fluid clearance in adult ventilated rats were studied under control conditions and after beta -adrenergic stimulation. Rats were instilled with a 5% albumin solution containing terbutaline (10(-4) M) or dibutyryl-cGMP (DBcGMP; 10-4 M) with or without the cyclic nucleotide-gated cation channel inhibitor l-cis-diltiazem (10(-3) M) and/or amiloride (10-3 M). Alveolar fluid clearance over 1 h was 18 +/- 2% in controls. In controls, amiloride inhibited 46 +/- 15% of alveolar fluid clearance, whereas l-cis-diltiazem had no inhibitory effect. Terbutaline and DBcGMP stimulated alveolar fluid clearance by 85 +/- 3 and 36 +/- 5%, respectively. Amiloride and l-cis-diltiazem inhibited nearly equal fractions of terbutaline-stimulated alveolar fluid clearance when given alone. Amiloride and l-cis-diltiazem given together inhibited a significantly larger fraction of alveolar fluid clearance in terbutaline-stimulated rats and in DBcGMP-stimulated rats. Based on these data, tertbutaline stimulation recruited both amiloride-sensitive and E-cis-diltiazem-sensitive pathways. In contrast, DBcGMP mainly recruited l-cis-diltiazem-sensitive pathways. Therefore, the amiloride-insensitive fraction of Nat-driven alveolar fluid clearance may be partly mediated through cyclic nucleotide-gated cation channels and activated by an increase in intracellular cGMP.

Subject

anesthetized sheep; cation channels; channels; conductance; cyclic nucleotide-gated cation channels; dibutyryl-guanosine-3'5'-cyclic monophosphate; distal lung; epithelial na+ channel; epithelial sodium ion; guinea-pigs; isoproterenol; l-cis-diltiazem; lung liquid clearance; Physiology; protein clearance; sodium transport; Sport Sciences; terbutaline

Format

Journal Article

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1489-1496

Issue

4

Volume

90

Citation

Norlin A; Lu L N; Guggino S E; Matthay M A; Folkesson H G, “Contribution of amiloride-insensitive pathways to alveolar fluid clearance in adult rats,” NEOMED Bibliography Database, accessed May 14, 2021, https://neomed.omeka.net/items/show/6654.

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