Histomorphometry of the embryonic avian growth plate by proton nuclear magnetic resonance microscopy

Title

Histomorphometry of the embryonic avian growth plate by proton nuclear magnetic resonance microscopy

Creator

Potter K; Landis W J; Spencer R G S

Publisher

Journal of Bone and Mineral Research

Date

2001
2001-06

Description

Quantitative nuclear magnetic resonance (NMR) microscopy was used to characterize the biochemical and morphological properties of the different zones within the growth plate of an embryonic chick femur. For precalcified tissue, water proton transverse relaxation times (T-2) and magnetization transfer values (MT) were directly and inversely dependent, respectively, on tissue cellularity, defined as the intracellular area per unit area on histological sections. T-2 values extrapolated for intra- and extracellular water were 96 ms and 46 ms, respectively. The extracellular T-2 was comparable with that measured for mature cartilage. The MT values extrapolated for intra- and extracellular compartments were 0.32 and 0.85, respectively, These values were comparable with those values reported in the literature for cell pellets and for mature cartilage tissue. Thus, cellularity dominated the NMR properties of this immature cartilage tissue. Mineral deposits within calcified cartilage and periosteal bone invoked NMR relaxation processes that were dependent on the inorganic mineral phase; Additionally, collagen molecules present in mineralized zones gave rise to a significant MT effect. These results show the utility of water proton NMR microscopy for assessing both the organic and inorganic ph ases within mineralized tissues.

Subject

appearance; articular-cartilage; bone-formation; cartilage; chick; deposits; diffusion; Endocrinology & Metabolism; growth-plate; hyaline cartilage; mineral; morphology; mr-imaging characteristics; nuclear magnetic resonance microscopy; relaxation; sequences

Format

Journal Article

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1092-1100

Issue

6

Volume

16

Citation

Potter K; Landis W J; Spencer R G S, “Histomorphometry of the embryonic avian growth plate by proton nuclear magnetic resonance microscopy,” NEOMED Bibliography Database, accessed July 24, 2021, https://neomed.omeka.net/items/show/6806.

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