Non-destructive studies of tissue-engineered phalanges by magnetic resonance microscopy and X-ray microtomography

Title

Non-destructive studies of tissue-engineered phalanges by magnetic resonance microscopy and X-ray microtomography

Creator

Potter K; Sweet D E; Anderson P; Davis G R; Isogai N; Asamura S; Kusuhara H; Landis W J

Publisher

Bone

Date

2006
2006-03

Description

One of the intents of tissue engineering is to fabricate biological materials for the augmentation or replacement of impaired, damaged, or diseased human tissue. In this context, novel models of the human phalanges have been developed recently through suturing of polymer scaffolds supporting osteoblasts, chondrocytes, and tenocytes to mimic bone, cartilage, and tendon, respectively. Characterization of the model constructs has been accomplished previously through histological and biochemical means, both of which are necessarily destructive to the constructs. This report describes the application of two complementary, non-destructive, non-invasive techniques, magnetic resonance microscopy (MRM) and X-ray microtomography (XMT or quantitative computed tomography), to evaluate the spatial and temporal growth and developmental status of tissue elements within tissue-engineered constructs obtained after 10 and 38 weeks of implantation in athymic (nude) mice. These two times represent respective points at which model middle phalanges are comprised principally of organic components while being largely unmineralized and later become increasingly more mineralized. The spatial distribution of mineralized deposits within intact constructs was readily detected by XMT (qCT) and was comparable to low intensity zones observed on MRM hydration maps. Moreover, the MRM-derived hydration values for mineralized zones were inversely correlated with mineral densities measured by XMT. In addition, the MRM method successfully mapped fat deposits, collagenous tissues, and the hydration state of the soft tissue elements comprising the specimens. These results support the application of non-destructive, non-invasive, quantitative MRM and XMT for the evaluation of constituent tissue elements within complex constructs of engineered implants. (c) 2005 Elsevier Inc. All rights reserved.

Subject

bone; bone-mineral density; cartilage; computed-tomography; computed-tomography; Endocrinology & Metabolism; in-vivo; macromolecules; magnetic resonance microscopy; microct; mr; quantitative; relaxation; scaffolds; tissue engineering; trabecular bone; X-ray microtomography

Format

Journal Article

Search for Full-text

Users with a NEOMED Library login can search for full-text journal articles at the following url: https://libraryguides.neomed.edu/home

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

350-358

Issue

3

Volume

38

Citation

Potter K; Sweet D E; Anderson P; Davis G R; Isogai N; Asamura S; Kusuhara H; Landis W J, “Non-destructive studies of tissue-engineered phalanges by magnetic resonance microscopy and X-ray microtomography,” NEOMED Bibliography Database, accessed December 1, 2021, https://neomed.omeka.net/items/show/6807.

Social Bookmarking