A novel early-stage orthotopic model for ovarian cancer in the Fischer 344 rat

Title

A novel early-stage orthotopic model for ovarian cancer in the Fischer 344 rat

Creator

Stakleff K D S; Rouse A G; Ryan A P; Haller N A; Von Gruenigen V E

Publisher

International Journal of Gynecological Cancer

Date

2005
2005-03

Description

The purpose of our study was to ascertain the progression of metastases in a novel ovarian cancer model designed to mimic early-stage disease by utilizing an orthotopic injection technique. Female Fischer 344 rats were injected with either 10(4) or 10(5) NuTu-19 cells by intraperitoneal or orthotopic injection. Peritoneal washings and histologic specimens were examined to correlate the incidence and extent of tumor growth. In a second phase, orthotopic injections of 10(2) and 10(3) cells were compared to that of 10(4) cells. Progression of ovarian cancer was observed by gross and microscopic examinations in both intraperitoneal and orthotopic models. Pelvic extension and abdominal adhesions uniquely characterized the orthotopically injected animals. Numbers of identifiable metastases declined with lower cell inocula, confirming that early-stage disease was extended to at least 14 days with 10(2) NuTu-19 cells. The orthotopic ovarian cancer model emulates early disease with the initiation of a primary tumor that is localized within the inherent microenvironment. The orthotopic model offers a clinically relevant alternative for future cancer research that allows for the investigation of therapeutic strategies against early stages of the disease process.

Subject

animal-model; epithelial neoplasms; Fischer 344 rats; gene-therapy; guinea-pigs; hormone; induction; mice; mouse model; Obstetrics & Gynecology; Oncology; orthotopic; ovarian-cancer; stimulation; tumors

Format

Journal Article

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

246-254

Issue

2

Volume

15

Citation

Stakleff K D S; Rouse A G; Ryan A P; Haller N A; Von Gruenigen V E, “A novel early-stage orthotopic model for ovarian cancer in the Fischer 344 rat,” NEOMED Bibliography Database, accessed October 17, 2021, https://neomed.omeka.net/items/show/7218.

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