A naturally derived dextran-peptide vector for microRNA antagomir delivery

A naturally derived dextran-peptide vector for microRNA antagomir delivery

Tang Q; Lei X; Cao B; Sun B B; Zhang Y Q; Cheng G

Rsc Advances

2015

2015

Single stranded microRNAs and their antagomirs are unstable and polyanionic, which impedes efficient cellular uptake and reduces half-life. Therefore, effective delivery systems with low toxicity for microRNAs are urgently needed for the success of microRNA-based therapy. Here, a dextran-peptide hybrid, Dex10-R5H5(40%), was developed as a carrier to deliver microRNAs. Dex10-R5H5(40%) loaded with antagomir-149 could reduce the level of endogenous microRNA-149 by 76% and it is more effective than the commercially available transfection reagent, RNAiMAX, which leads to 67% reduction. Additionally, Dex10-R5H5(40%) exhibited no cytotoxicity to HepG2 cells. These results indicate that the dextran-peptide hybrid may be a promising delivery system for the safe and efficient microRNA-based therapy.

cells; Chemistry; gene delivery; human cancers; in-vitro; mechanism; RNA interference; therapy; vivo

Journal Article

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

28019-28022

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