Identification of Multiple Metabolic Enzymes from Mice Cochleae Tissue Using a Novel Functional Proteomics Technology
Title
Identification of Multiple Metabolic Enzymes from Mice Cochleae Tissue Using a Novel Functional Proteomics Technology
Creator
Wang D L; Li H; Liang R Q; Bao J X
Publisher
PLOS ONE
Date
2015
2015-03
Description
A new type of technology in proteomics was developed in order to separate a complex protein mixture and analyze protein functions systematically. The technology combines the ability of two-dimensional gel electrophoresis (2-DE) to separate proteins with a protein elution plate (PEP) to recover active proteins for functional analysis and mass spectrometry (MS)-based identification. In order to demonstrate the feasibility of this functional proteomics approach, NADH and NADPH-dependent oxidases, major redox enzyme families, were identified from mice cochlear tissue after a specific drug treatment. By comparing the enzymatic activity between mice that were treated with a drug and a control group significant changes were observed. Using MS, five NADH-dependent oxidases were identified that showed highly altered enzymatic activities due to the drug treatment. In essence, the PEP technology allows for a systematic analysis of a large enzyme family from a complex proteome, providing insights in understanding the mechanism of drug treatment.
Subject
Animal tissues; Beef; Biology; cancer; cancer metabolism; cell metabolism; Cochlea; draft; Drug therapy; E coli; Electrophoresis; Elution; Enzymatic activity; Enzyme assays; Enzyme metabolism; Enzymes; Feasibility studies; Functional analysis; Gel electrophoresis; Genes; Genomics; Hearing impairment; Kinases; Mass spectrometry; Mass spectroscopy; medicine; messenger-rna; metabolism; mice; NAD(P)H oxidase; NADH; Neurobiology; Neurosciences; Nicotinamide adenine dinucleotide; Noise; Otolaryngology; Oxidation-reduction reactions; Phosphatase; Protein expression; proteins; Proteomes; Proteomics; Science & Technology - Other Topics; Sciences: Comprehensive Works; Scientific imaging; Studies; Substance abuse treatment; Technology; United States--US
Identifier
Format
Journal Article
URL Address
Search for Full-text
Users with a NEOMED Library login can search for full-text journal articles at the following url: https://libraryguides.neomed.edu/home
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
13-13
Issue
3
Volume
10
Citation
Wang D L; Li H; Liang R Q; Bao J X, “Identification of Multiple Metabolic Enzymes from Mice Cochleae Tissue Using a Novel Functional Proteomics Technology,” NEOMED Bibliography Database, accessed February 13, 2025, https://neomed.omeka.net/items/show/7449.