Simplified method for concentration of mitochondrial membrane protein complexes
Title
Simplified method for concentration of mitochondrial membrane protein complexes
Creator
Yeh S T; Angelos M G; Chen Y R
Publisher
Electrophoresis
Date
2010
2010-06
Description
Extracting and concentrating mitochondrial protein complexes from gel strips after blue native PAGE (BN-PAGE) can be daunting tasks using the traditional methods, such as electroelution, passive diffusion and centrifugal concentration. We present a simplified gel electrophoresis method to concentrate mitochondrial protein complexes with excellent recovery rate. Mitochondrial complex I present in a long gel strip from BN-PAGE can be easily concentrated into a 0.8 cm gel strip when a second BN-PAGE is performed with a Y-shaped gel and the addition of 0.01% n-dodecyl beta-D-maltoside and 0.001% SDS in the cathode buffer. Once completed, the concentrated protein complex in the gel strip is ready for SDS-PAGE or proteomic studies.
Subject
Biochemistry & Molecular Biology; blue; Blue native PAGE; Chemistry; Complex I; Electrophoretic concentration; Mitochondria; native electrophoresis; oxidative-phosphorylation; polyacrylamide-gels; sds-page
Identifier
Format
Journal Article
URL Address
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Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1934-1936
Issue
12
Volume
31
Citation
Yeh S T; Angelos M G; Chen Y R, “Simplified method for concentration of mitochondrial membrane protein complexes,” NEOMED Bibliography Database, accessed April 22, 2025, https://neomed.omeka.net/items/show/7638.