Simplified method for concentration of mitochondrial membrane protein complexes

Title

Simplified method for concentration of mitochondrial membrane protein complexes

Creator

Yeh S T; Angelos M G; Chen Y R

Publisher

Electrophoresis

Date

2010
2010-06

Description

Extracting and concentrating mitochondrial protein complexes from gel strips after blue native PAGE (BN-PAGE) can be daunting tasks using the traditional methods, such as electroelution, passive diffusion and centrifugal concentration. We present a simplified gel electrophoresis method to concentrate mitochondrial protein complexes with excellent recovery rate. Mitochondrial complex I present in a long gel strip from BN-PAGE can be easily concentrated into a 0.8 cm gel strip when a second BN-PAGE is performed with a Y-shaped gel and the addition of 0.01% n-dodecyl beta-D-maltoside and 0.001% SDS in the cathode buffer. Once completed, the concentrated protein complex in the gel strip is ready for SDS-PAGE or proteomic studies.

Subject

Biochemistry & Molecular Biology; blue; Blue native PAGE; Chemistry; Complex I; Electrophoretic concentration; Mitochondria; native electrophoresis; oxidative-phosphorylation; polyacrylamide-gels; sds-page

Format

Journal Article

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

1934-1936

Issue

12

Volume

31

Citation

Yeh S T; Angelos M G; Chen Y R, “Simplified method for concentration of mitochondrial membrane protein complexes,” NEOMED Bibliography Database, accessed March 4, 2021, https://neomed.omeka.net/items/show/7638.

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