INSITU HYBRIDIZATION STUDY OF OBESITY-ASSOCIATED ALTERATION IN GROWTH-HORMONE MESSENGER-RNA LEVELS

Title

INSITU HYBRIDIZATION STUDY OF OBESITY-ASSOCIATED ALTERATION IN GROWTH-HORMONE MESSENGER-RNA LEVELS

Creator

Ahmad I; Steggles A W; Finkelstein J A

Publisher

International Journal of Obesity

Date

1992
1992-06

Description

In order to investigate whether the impaired GH secretion associated with obesity is due to a pituitary disorder we studied GH mRNA levels by in situ hybridization in genetically obese and lean Zucker rats. The levels of GH mRNA were at least two fold lower in obese rats in comparison to that in lean controls as quantified by both the scanning of autoradiographs of tissue sections and Northern blot analysis. Quantification of somatotrophs revealed no significant difference in their number between lean and obese rat pituitaries. It is therefore likely that the attenuated GH mRNA levels in genetically obese Zucker rats are due to a decrease in GH transcripts per somatotroph rather than a result of a pituitary defect involving a preferential decrease in somatotroph population.

Subject

differentiation; Endocrinology & Metabolism; expression; fetal-rat; gene-transcription; glucocorticoid hormones; growth hormone; insitu hybridization; insulin; lean zucker rats; messenger-rna; Nutrition & Dietetics; pituitary-tumor cells; ribonucleic-acid; triiodothyronine

Identifier

n/a

Format

Journal Article or Conference Abstract Publication

URL Address

n/a

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

435-441

Issue

6

Volume

16

Citation

Ahmad I; Steggles A W; Finkelstein J A, “INSITU HYBRIDIZATION STUDY OF OBESITY-ASSOCIATED ALTERATION IN GROWTH-HORMONE MESSENGER-RNA LEVELS,” NEOMED Bibliography Database, accessed May 29, 2022, https://neomed.omeka.net/items/show/8436.

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