Regulation of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7A): Prevalence of negative over positive transcriptional control

Title

Regulation of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7A): Prevalence of negative over positive transcriptional control

Creator

DeFabiani E; Crestani M; Marrapodi M; Pinelli A; Chiang J Y L; Galli G

Publisher

Biochemical and Biophysical Research Communications

Date

1996
1996-09

Description

Cholesterol 7 alpha-hydroxylase plays a crucial role in cholesterol homeostasis. We investigated the regulation of this enzyme in the hamster, a suitable animal model for studying cholesterol metabolism. DNase I hypersensitivity assay revealed the presence of a hypersensitive region in the proximal promoter. Both negative (bile acids, phorbol esters and insulin) and positive (glucocorticoid hormones) effects were mediated through sequences in the region 318 bp upstream of the ATG codon. All-trans-retinoic acid, cAMP, and LDL did not affect transcriptional activity. These findings show that the hamster cholesterol 7 alpha-hydroxylase gene undergoes a predominant negative regulation, as opposed to the rat CYP7A homologous gene. (C) 1996 Academic Press, Inc.

Subject

Biophysics; down-regulation; Biochemistry & Molecular Biology; hmg-coa reductase; messenger-rna levels; Bile acids; protein-kinase-c; cultures; primary; thyroid-hormone; rat hepatocytes; hormonal-regulation; hypophysectomized rats

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

663-671

Issue

3

Volume

226

Citation

DeFabiani E; Crestani M; Marrapodi M; Pinelli A; Chiang J Y L; Galli G, “Regulation of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7A): Prevalence of negative over positive transcriptional control,” NEOMED Bibliography Database, accessed June 16, 2021, https://neomed.omeka.net/items/show/9037.

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