miR-145 is differentially regulated by TGF-ss 1 and ischaemia and targets Disabled-2 expression and wnt/ss-catenin activity
Title
miR-145 is differentially regulated by TGF-ss 1 and ischaemia and targets Disabled-2 expression and wnt/ss-catenin activity
Creator
Mayorga M E; Penn M S
Publisher
Journal of Cellular and Molecular Medicine
Date
2012
2012-05
Description
The effect of wnt/beta-catenin signalling in the response to acute myocardial infarction (AMI) remains controversial. The membrane receptor adaptor protein Disabled-2 (Dab2) is a tumour suppressor protein and has a critical role in stem cell specification. We recently demonstrated that down-regulation of Dab2 regulates cardiac protein expression and wnt/beta-catenin activity in mesenchymal stem cells (MSC) in response to transforming growth factor-beta 1 (TGF-beta 1). Although Dab2 expression has been shown to have effects in stem cells and tumour suppression, the molecular mechanisms regulating this expression are still undefined. We identified putative binding sites for miR-145 in the 3'-UTR of Dab2. In MSC in culture, we observed that TGF-beta 1 treatment led to rapid and sustained up-regulation of primiR-145. Through gain and loss of function studies we demonstrate that miR-145 up-regulation was required for the down-regulation of Dab2 and increased beta-catenin activity in response to TGF-beta 1. To begin to define how Dab2 might regulate wnt/beta-catenin in the heart following AMI, we quantified myocardial Dab2 as a function of time after left anterior descending ligation. There was no significant Dab2 expression in sham-operated myocardium. Following AMI, Dab2 levels were rapidly up-regulated in cardiac myocytes in the infarct border zone. The increase in cardiac myocyte Dab2 expression correlated with the rapid and sustained down-regulation of myocardial primiR-145 expression following AMI. Our data demonstrate a novel and critical role for miR-145 expression as a regulator of Dab2 expression and beta-catenin activity in response to TGF-beta 1 and hypoxia.
Subject
gene-expression; Myocardial infarction; Cell Biology; Myocardial infarction; Research & Experimental Medicine; stem-cells; binding; pathway; cytokines; growth factors; c-myc; cell-differentiation; dab2; epithelial ovarian-cancer; myosin-vi; nasopharyngeal carcinoma
Identifier
Format
Journal Article or Conference Abstract Publication
URL Address
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Rights
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Pages
1106-1113
Issue
5
Volume
16
Citation
Mayorga M E; Penn M S, “miR-145 is differentially regulated by TGF-ss 1 and ischaemia and targets Disabled-2 expression and wnt/ss-catenin activity,” NEOMED Bibliography Database, accessed May 30, 2023, https://neomed.omeka.net/items/show/9050.