Measurement Of Reactive Oxygen Species, Reactive Nitrogen Species, And Redox-dependent Signaling In The Cardiovascular System: A Scientific Statement From The American Heart Association

Title

Measurement Of Reactive Oxygen Species, Reactive Nitrogen Species, And Redox-dependent Signaling In The Cardiovascular System: A Scientific Statement From The American Heart Association

Creator

Griendling K K; Touyz R M; Zweier J L; Dikalov S; Chilian W; Chen Y R; Harrison D G; Bhatnagar A; Amer Heart Assoc Council Basic

Publisher

Circulation Research

Date

2016
2016-08

Description

Reactive oxygen species and reactive nitrogen species are biological molecules that play important roles in cardiovascular physiology and contribute to disease initiation, progression, and severity. Because of their ephemeral nature and rapid reactivity, these species are difficult to measure directly with high accuracy and precision. In this statement, we review current methods for measuring these species and the secondary products they generate and suggest approaches for measuring redox status, oxidative stress, and the production of individual reactive oxygen and nitrogen species. We discuss the strengths and limitations of different methods and the relative specificity and suitability of these methods for measuring the concentrations of reactive oxygen and reactive nitrogen species in cells, tissues, and biological fluids. We provide specific guidelines, through expert opinion, for choosing reliable and reproducible assays for different experimental and clinical situations. These guidelines are intended to help investigators and clinical researchers avoid experimental error and ensure high-quality measurements of these important biological species.

Subject

acute myocardial-infarction; AHA Scientific Statements; Cardiovascular System & Cardiology; coronary-artery-disease; free radicals; Hematology; ischemia-reperfusion injury; liquid-chromatography; low-density-lipoprotein; measurements; nitric-oxide synthase; nitrogen species; oxidants; oxidative stress; performance; peroxides; protein-tyrosine phosphatases; reactive; reactive oxygen species; tandem mass-spectrometry; vascular smooth-muscle

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

E39-E75

Issue

5

Volume

119

Citation

Griendling K K; Touyz R M; Zweier J L; Dikalov S; Chilian W; Chen Y R; Harrison D G; Bhatnagar A; Amer Heart Assoc Council Basic, “Measurement Of Reactive Oxygen Species, Reactive Nitrogen Species, And Redox-dependent Signaling In The Cardiovascular System: A Scientific Statement From The American Heart Association,” NEOMED Bibliography Database, accessed January 24, 2021, https://neomed.omeka.net/items/show/10012.

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