Orphan Nuclear Receptor Small Heterodimer Partner Negatively Regulates Growth Hormone-mediated Induction Of Hepatic Gluconeogenesis Through Inhibition Of Signal Transducer And Activator Of Transcription 5 (stat5) Transactivation

Title

Orphan Nuclear Receptor Small Heterodimer Partner Negatively Regulates Growth Hormone-mediated Induction Of Hepatic Gluconeogenesis Through Inhibition Of Signal Transducer And Activator Of Transcription 5 (stat5) Transactivation

Creator

Kim Y D; Li T G; Ahn S W; Kim D K; Lee J M; Hwang S L; Kim Y H; Lee C H; Lee I K; Chiang J Y L; Choi H S

Publisher

Journal of Biological Chemistry

Date

2012
2012-10

Description

Growth hormone (GH) is a key metabolic regulator mediating glucose and lipid metabolism. Ataxia telangiectasia mutated (ATM) is a member of the phosphatidylinositol 3-kinase superfamily and regulates cell cycle progression. The orphan nuclear receptor small heterodimer partner (SHP: NR0B2) plays a pivotal role in regulating metabolic processes. Here, we studied the role of ATM on GH-dependent regulation of hepatic gluconeogenesis in the liver. GH induced phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase gene expression in primary hepatocytes. GH treatment and adenovirus-mediated STAT5 overexpression in hepatocytes increased glucose production, which was blocked by a JAK2 inhibitor, AG490, dominant negative STAT5, and STAT5 knockdown. We identified a STAT5 binding site on the PEPCK gene promoter using reporter assays and point mutation analysis. Up-regulation of SHP by metformin-mediated activation of the ATM-AMP-activated protein kinase pathway led to inhibition of GH-mediated induction of hepatic gluconeogenesis, which was abolished by an ATM inhibitor, KU-55933. Immunoprecipitation studies showed that SHP physically interacted with STAT5 and inhibited STAT5 recruitment on the PEPCK gene promoter. GH-induced hepatic gluconeogenesis was decreased by either metformin or Ad-SHP, whereas the inhibition by metformin was abolished by SHP knockdown. Finally, the increase of hepatic gluconeogenesis following GH treatment was significantly higher in the liver of SHP null mice compared with that of wildtype mice. Overall, our results suggest that the ATM-AMP-activated protein kinase-SHP network, as a novel mechanism for regulating hepatic glucose homeostasis via a GH-dependent pathway, may be a potential therapeutic target for insulin resistance.

Subject

ampk; ataxia-telangiectasia; atm; Biochemistry & Molecular Biology; expression; Glucose; glucose-6-phosphatase gene; involvement; metformin; protein kinase; shp

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

37098-37108

Issue

44

Volume

287

Citation

Kim Y D; Li T G; Ahn S W; Kim D K; Lee J M; Hwang S L; Kim Y H; Lee C H; Lee I K; Chiang J Y L; Choi H S, “Orphan Nuclear Receptor Small Heterodimer Partner Negatively Regulates Growth Hormone-mediated Induction Of Hepatic Gluconeogenesis Through Inhibition Of Signal Transducer And Activator Of Transcription 5 (stat5) Transactivation,” NEOMED Bibliography Database, accessed June 14, 2021, https://neomed.omeka.net/items/show/10478.

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