Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling
Title
Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling
Creator
Vincent Serapiglia
Chad A Stephens
Rashika Joshi
Emrah Aydin
Marc Oria
Mario Marotta
Jose L Peiro
Brian M Varisco
Date
2022
Description
Fetal endoscopic tracheal occlusion (FETO) is an emerging surgical therapy for congenital diaphragmatic hernia (CDH). Ovine and rabbit data suggested altered lung epithelial cell populations after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells. To test this hypothesis, we deconvolved mRNA sequencing (mRNA-seq) data and used quantitative image analysis in fetal rabbit lung TO, which had increased basal cells and reduced ciliated cells after TO. In a fetal mouse TO model, flow cytometry showed increased basal cells, and immunohistochemistry demonstrated basal cell extension to subpleural airways. Nuclear Yap, a known regulator of basal cell fate, was increased in TO lung, and Yap ablation on the lung epithelium abrogated TO-mediated basal cell expansion. mRNA-seq of TO lung showed increased activity of downstream Yap genes. Human lung specimens with congenital and fetal tracheal occlusion had clusters of subpleural basal cells that were not present in the control. TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.
Source
Front Pediatr
. 2022 Feb 22;9:780166. doi: 10.3389/fped.2021.780166. eCollection 2021.
. 2022 Feb 22;9:780166. doi: 10.3389/fped.2021.780166. eCollection 2021.
Language
English
URL
https://doi.org/10.3389/fped.2021.780166
Citation
Vincent Serapiglia et al., “Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling,” NEOMED Bibliography Database, accessed May 1, 2024, https://neomed.omeka.net/items/show/12080.