Characterization Of Regional Cerebral Blood Flow And Expression Of Angiogenic Growth Factors In The Frontal Cortex Of Juvenile Male Shrsp And Shr

Title

Characterization Of Regional Cerebral Blood Flow And Expression Of Angiogenic Growth Factors In The Frontal Cortex Of Juvenile Male Shrsp And Shr

Creator

Jesmin S; Togashi H; Mowa C N; Ueno K; Yamaguchi T; Shibayama A; Miyauchi T; Sakuma I; Yoshioka M

Publisher

Brain Research

Date

2004
2004-12

Description

Attention-deficit/hyperactivity disorder (AD/HD) is a common pediatric behavioral disorder associated with male preponderance and reduction of regional cerebral blood flow (rCBF). However, lack of an appropriate animal model exhibiting appropriate AD/HD symptoms stands in the way of studying mechanism(s) underlying reduced rCBF and male preponderance. Our group has been investigating the suitability of juvenile male stroke-prone spontaneously hypertensive rats (SHRSP), a substrain of the commonly used AD/HD animal model SHR, as a model for AD/HD because, unlike SHR, SHRSP displays cognitive impairment and male preponderance. Our more recent studies revealed alterations in the synthesis of sex steroid hormones and angiogenic factors in the frontal cortex of male SHRSP compared to the genetic control WKY. Based on these observations, the present study utilizes laser-Doppler flowmetry, histochemistry, enzyme immunoassay, immunoblotting, and real-time PCR to characterize and compare the patterns of regional cerebral blood flow and synthesis of angiogenic molecules [basic fibroblast growth factor; nitric oxide synthase isoforms (endothelial, neuronal and inducible); vascular endothelial growth factor (VEGF) and its signaling molecules VEGF receptors, phosphorylated Akt, endothelial nitric oxide synthase eNOS] between male SHRSP and SHR. Overall, consistent with our previous data showing alteration in VEGF/Akt/NO signaling, there was a marked reduction in the profile of rCBF (35%) and angiogenic factors of SHRSP, compared to age-matched genetic control Wistar-Kyoto rats (WKY) and SHR. We conclude that, unlike SHR, the profiles of rCBF and angiogenic factors in SHRSP are altered in juvenile male. Thus, SHRSP appears to be a more suitable animal model for studying changes in rCBF in AD/HD. (C) 2004 Elsevier B.V. All rights reserved.

Subject

abnormalities; AD/HD; angiogemic factor; animal-model; attention-deficit/hyperactivity; brain; children; deficit-hyperactivity-disorder; disorder; frontal cortex; Neurosciences & Neurology; nitric-oxide; NOS isoform; regional cerebral blood flow; spontaneously hypertensive-rats; stroke-prone; vegf

Format

Journal Article or Conference Abstract Publication

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Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

172-182

Issue

2

Volume

1030

Citation

Jesmin S; Togashi H; Mowa C N; Ueno K; Yamaguchi T; Shibayama A; Miyauchi T; Sakuma I; Yoshioka M, “Characterization Of Regional Cerebral Blood Flow And Expression Of Angiogenic Growth Factors In The Frontal Cortex Of Juvenile Male Shrsp And Shr,” NEOMED Bibliography Database, accessed April 17, 2021, https://neomed.omeka.net/items/show/10353.

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