Specific And Efficient Transduction Of Cochlear Inner Hair Cells With Recombinant Adeno-associated Virus Type 3 Vector

Title

Specific And Efficient Transduction Of Cochlear Inner Hair Cells With Recombinant Adeno-associated Virus Type 3 Vector

Creator

Liu Y H; Okada T; Sheykholeslami K; Shimazaki K; Nomoto T; Muramatsu S I; Kanazawa T; Takeuchi K; Ajalli R; Mizukami H; Kume A; Ichimura K; Ozawa K

Publisher

Molecular Therapy

Date

2005
2005-10

Description

Recombinant adeno-associated virus (AAV) vectors are of interest for cochlear gene therapy because of their ability to mediate the efficient transfer and long-term stable expression of therapeutic genes in a wide variety of postmitotic tissues with minimal vector-related cytotoxicity. In the present study, seven AAV serotypes (AAV1-5, 7, 8) were used to construct vectors. The expression of EGFP by the chicken P-actin promoter associated with the cytomegalovirus immediate-early enhancer in cochlear cells showed that each of these serotypes successfully targets distinct cochlear cell types. In contrast to the other serotypes, the AAV3 vector specifically transduced cochlear inner hair cells with high efficiency in vivo, while the AAV1, 2, 5, 7, and 8 vectors also transduced these and other cell types, including spiral ganglion and spiral ligament cells. There was no loss of cochlear function with respect to evoked auditory brain-stem responses over the range of frequencies tested after the injection of AAV vectors. These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells.

Subject

adeno-associated virus; adenovirus; Biotechnology & Applied Microbiology; cochlea; ear; Experimental Medicine; gene transfer; gene transfer; generation; Genetics & Heredity; guinea-pig cochlea; hair cells; in-vivo; promoter; rat; Research &; serotype; therapy; transgene expression

Format

Journal Article or Conference Abstract Publication

Search for Full-text

Users with a NEOMED Library login can search for full-text journal articles at the following url: https://libraryguides.neomed.edu/home

Rights

Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).

Pages

725-733

Issue

4

Volume

12

Citation

Liu Y H; Okada T; Sheykholeslami K; Shimazaki K; Nomoto T; Muramatsu S I; Kanazawa T; Takeuchi K; Ajalli R; Mizukami H; Kume A; Ichimura K; Ozawa K, “Specific And Efficient Transduction Of Cochlear Inner Hair Cells With Recombinant Adeno-associated Virus Type 3 Vector,” NEOMED Bibliography Database, accessed April 11, 2021, https://neomed.omeka.net/items/show/10782.

Social Bookmarking