Multiple mechanisms for HSV-1 induction of interferon alpha production by peripheral blood mononuclear cells.

Multiple mechanisms for HSV-1 induction of interferon alpha production by peripheral blood mononuclear cells.

Rong Q; Alexander T S; Koski G K; Rosenthal K S

Archives of virology

2003

2003-02

UV-inactivated, infectious, and other forms of herpes simplex virus 1 (HSV-1) induced interferon (IFN) production by different routes in myeloid origin mononuclear cells (MOMC) (consisting predominantly of monocytes). GM-CSF activated the MOMC (G-MOMC) to produce greater amounts of interferon while differentiation to DC, by the addition of granulocyte macrophage colony stimulating factor (GM-CSF) and calcium ionophore (GA-MOMC), reduced the levels of interferon production upon challenge with some HSV strains. UV-inactivated virus induced more interferon than infectious virus. L-fucose, an antagonist of the mannose receptor, inhibited the induction of IFN-alpha by UV-inactivated virus and gB(-) virus (defective in penetration) in MOMC and GA-MOMC but not

Fucose/pharmacology; Gene Expression Regulation/drug effects; Herpesvirus 1; Human/classification/drug effects/genetics/*physiology; Humans; Interferon-alpha/*biosynthesis; Mutation; Myeloid Cells/drug effects/*metabolism/*virology; Species Specificity; Virus Replication

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